※Enrichment culture(집적배양)
미생물학자 Martinus Beijerinck에 의해 처음 시도된 방법으로 aerobic N2-fixing bacterium Azotobacter 를 사용하여 배양하였다.
selection for aerobic N₂-fixing bacteria usually results
in the isolation of Azotobacter or its relatives. By contrast,
enrichment with fixed forms of nitrogen such as NH₄+
rarely results in N₂-fixing
culture
b. Identification of Gram reaction : 사용할 카드를 선별하기 위해(GN or GP card)
c. Dilution of bacteria : test에 사용하기 적절한 탁도로 균을 희석
멸균된 유리관에 0.45% saline을 3ml씩 분주한 후 멸균된 면봉을 이용하여 colony를
picking하여 유리관 내 saline에 희석한다. 이 후 spectrophotometer를 이용하여 0
culture is usually sustained on gel media, in much the same manner as bacteria are grown. Sufficient media consist of agar and the usual mix of macronutrients and micronutrients for the given cell type. For plant cells, enrichment with nitrogen, phosphorus, and potassium is especially important. Water is provided as a constituent part of the gel media.
Uses
A plant cell callus consists of somat
The manufacturing process of the lactic fermented milk
The manufacturing process of the lactic fermented milk is classified as following below.
1. Manufacturing process of milk (Up & Midstream.)
Skim milk (milk) puts into dissolution tank and U.H.P(a high temperature sterilizer) then transfer into cultivation tank. It transfers to be homogeneous and remove the microorganism aga
culture but Norway has transformed its economy over the past decades into a modern industry. Now the oil and gas, maritime and the seafood sectors are the most remarkable industries in Norway. Above these, the energy & environment, healthcare and green IT are emerging industries. From now on look at these three important industries in Norway and then others in Norway more specifically.
Paragrap
1. Geographic Characteristics
Name
- The Socialist Republic of Vietnam
Capital
- Ha Noi
Climate
- subtropical(Northern), tropical(Southern)
Environment
- From May to January, there are season
typhoons that occur deluge
2. Health Status
1) Facilities
▶ Expanding city-centered hospitals and private medical institution
→ give medical services of high quali
1. 서론
Prokaryote란 원핵생물을 뜻하는 말로써 Carl Woese 에 의해 새로 도입된 분류법의 3가지 Domain - Archaea, Bacteria, Eukaryota - 중 진핵생물인 Eukaryota를 제외한 생물을 지칭하는 말이다. 진핵생물과는 달리 특징적으로 핵막이 없는 원핵생물은 단세포생물일 경우가 많으며 박테리아나 같은 원핵생물 및 주
Cell Culture
∙ What is cell culture?
생물체의 조직편이나 조직에서 분리된 세포들을 생물체내의 환경과 동일한 배양시스템에서 유지시켜 줌으로써 조직이나 세포의 원래 기능을 보유하도록 하는 기술의 한 방법
∙ 동결보존이 필요한 이유
주세포의 경우 계대배양 중에 세포 모양이나 성질이
market, we, 美ME+ also is looking to expand our business.
This report is divided into two parts, the first half; we will talk about the Japanese market as a whole cultural risk, country risk, and currency risk. And in the later half; we will analysis the cosmetics market in Japan, and describe our company’s market strategy. After that, we will predict future prospects of our company.
Experiment #1: Contamination
Principle
미생물은 우리 눈에 보이지는 않지만 우리가 생활 하는 주위 곳곳에 살고 있다. 우리가 숨을 들이쉬고 내쉬는 공기와 우리가 일상적으로 만지고 사용하는 물체와 심지어 우리의 손에도 미생물은 존재한다. 이것은 실험을 할 때에 무균조작법(aseptic technique)의 중요성을 깨